Biotechnology Syllabus

Some of the things we will cover include:

 

UNIT

CONTENTS

SKILLS

Introduction to Biotechnology

 

A Historical Framework of the Biotechnology Industry.

.

Course Syllabus & Textbook.

Rules and Expectations.

Lab Notebooks:

  • Concepts, Protocols, & Reflections.
  • Flow Charts

Lab Safety

  • Safety contract.

Concepts and Reflections Journaling

 

“Tools of the trade”

 

LABs:

Micropipetting

Made to Measure

Dilutions and solutions

 

 

Micropipetting

Scale and measurement.

“More Tools of the trade”

 

DNA Theory:

  • Central dogma review.

 

Serial Dilution of Bacteria

DNA Extraction:

  • The Stuff Genes are Made of

Electrophoresis:

  • Electrophoresis exploration
  • Dye indicator lab
  • DNA Lab1 “Whodunnit
  • DNA Lab 2 “Cut and Paste”

 

DNA Extraction

Bacterial growth

DNA Vectors

  • Plasmids
  • Viral

Electrophoresis

  • Restriction Enzymes Plasmid/ Restriction Digests

 

Serial dilution

Sterile Technique

 

Genome Biotechnology

‘Bioinformatics

  • Bioinformatics and the Human Genome Project
  • Genome Exploration, A Web-based approach.

Methods for finding and expressing important genes.

The Human Genome Project

DNA Sequencing Techniques

Bioinformatics

 

BLAST Searches

Genbank

Structural Biology

The Structure, Genetics, Function, and Regulation of DNA and Proteins

 

 

PGLO/pBLUTransformation

  • “Genes are Us”

Molecular Chromatography

  • “Molecular Mixtures”

Transformation and Recombinant Organisms.

Transcriptional control

Operons

RNAi

 

Bacterial Transformation

Chromatography

  • Protein purification

 

Transformation

Chromatography

 

Other elements of the Industry.

Manufacturing and QC

Imupop

Process Scale-up

Recovery

Large Scale Manufacturing

Sales and Marketing

Legal (Intellectual Property)

 

 

Large scale manufacturing and Quality Control Issues.

 

TOPICS FOR AN EXPANDED SYLLABUS

 

 

 

Biotechnology and Society

 

Bioethics

Human Genetic Disease

Evolution

 

 

PCR and Forensics

PCR Techniques

Forensic applications

Human Allelic Variation

 

Immunology and Biotechnology

The Immune System and Antibody Production

Monoclonal antibodies

The Western Blot and Immunoassays

Protein Conformation and Modeling

 

 

The Science of Cancer

 

The faces of Cancer

Cancer and the cell cycle

 

Drosophila Molecular Genetics

 

 

The Molecular Genetics of AIDS