Biotech Spring 2019

DATE	CLASS ACTIVITY	ASSIGNMENTS
Mon Feb 4	Semester 2 Start SNOW DAY.
5	Introduction to Class. Class Info and Expectations.doc	3 Ring Binder for classroom ASAP Prokaryotic vs Eukaryotic Assignment.doc Due Friday at the beginning of class
6	Who are you: Introductions Hartman Personality profile.doc Colors decoded.doc
7	UNIT 1 : DNA Structure Answer the Q. What is DNA? What is Biotechnology.ppt Intro to DNA Webquest.doc
Fri Feb 8	Pro v Euk assignment due DNA Carries information...Some Notes: DNA is the information Molecule.ppt Student notes sheet.doc Start DNA model building. Early release for Snomageddon
Mon Feb 11	SNOWMAGEDDON cont.
12	SNOWMAGEDDON cont.
13	SNOWMAGEDDON cont.
14	SNOWMAGEDDON cont.
Fri 15	SNOWMAGEDDON cont.
Mon Feb 18	Presidents Day
Tues 19	DNA is the information Molecule.ppt DNA Model Cont.
20	Finish Notes Draw the carbon backbone of Deoxyribose. 5', 3', and Antiparallel explained DNA Dance.doc Finish Model
21	The Structure of DNA.ppt The Structure of DNA.notes DNA Structure readymade notes.doc
Fri 22	Finish Notes DNA Structure Quick Quiz.doc
Mon 25	DNA Structure Test
26	Math in the LAB .doc (Yes Math!!!)
27	Biotechnology Tools I: Micropipettes Introduction to Micropipettes Micropipettes 1: Measure for Measure LAB
28	Finish Measure for measure
Fri Mar 1	Micropipettes 2: Color Wheel LAB	Micropipette CPR Rubric.doc CPR example on Gravity written by Dr. F.docx --Due Wed. Mar 13
Mon Mar 4	Micropipettes 3: Micropipette Challenge LAB
5	Finish Challenge LAB
6	Micropipettes 4: Serial Dilutions Lab Serial Dilution Worksheet.doc
7	SNOW DAY :-(
Fri 8	Serial Dilution and Worksheet	Paid Summer Internship at the PNW Salmon Center (Belfair)
Mon 11	Pre Lab for Bacterial Serial Dilution Finish Worksheet
12	Bacterial Serial Dilution
13	CPR DUE Bacteria Counting and Data analysis
14	Unit 2: DNA Function Part 1... DNA functions to replicate itself. DNA Replication.ppt DNA Replication Notes scaffold.doc DNA Replication Picture.doc	Concept I: DNA replication is Semi Conservative Last Chance to Finish up all LABS and worksheets Bozeman on the Meselson and Stahl experiment: Video
Fri 15	DNA Replication Notes	Concept I: DNA replication is Semi Conservative. Concept II: Replication always proceeds from a 5'->3' direction
Mon 18	Finish DNA Replication	Concept III : Replication occurs simultaneously from multiple origins. Concept IV: Replication requires many enzymes.
19	DNA Extraction LAB
20	Finish DNA Extraction LAB. Review DNA Replication Quiz.doc Quiz Key.doc
21	DNA Replication Test
Fri 22	Start Electrophoresis explorationI	Electrophoresis CPR DUE 1 concept (a minimum of 3x150 word paragraphs each) 2 reflections (100 words each min) Typed, 12 point font. double spaced
Mon 25	Finish Electrophoresis Exploration I
26	Electrophoresis exploration II Practice Pouring and Loading gels
27	Electrophoresis exploration II Practice Pouring, Loading gels, and sample set-up
28	Run Gel and get results
Fri 29	Catch up, CPR	No missing work (including re-writes or Lab worksheets) will be accepted after Today!
Mon. April 1st - Fri 5th	SPRING BREAK
Mon April 8	Candy Lab
9	Candy Lab
10	Electrophoresis CPR DUE DNA Function 2: Carrying the Code Part I: Elements of Transcription. Q. What is a gene? Introduction to Transcription.ppt Transcription Notes & Worksheet.doc Transcription Vocabulary.doc Complete Lambda DNA Sequence.doc
11	Finish Elements of Transcription RNA and Transcription.ppt Transcription animation.ppt Animation Notes.doc	DNAi Transcription Video Scribe and Chef analogy video
Fri 12	Part II: Elements of Translation. Introduction to translation.ppt the code of translation.ppt Translation animation.ppt student animation notes scaffold.doc Student translation vocab.doc Translation notes/practice 1.doc Translation Notes/practice 2.doc http://www.youtube.com/watch?v=H5udFjWDM3E
Mon 15	FINISH Translation notes & practice worksheets
16	Dingo Secret message.doc
17	DNA Mutations.ppt Mutations practice.doc	No Late Work after today Bozeman on Mutations Video Bozeman Mutation Quiz.docx
18	Review Ribosome and transcription Practice Diagram.doc DNA Function Vocab Quiz.doc DNA Function II - Complete Vocab with Definitions.doc Transcription/translation practice Quiz.doc Transcription/translation practice Test.pdf	Paul Berg Translation Dance Video Twas Brillig Lyrics.doc
Fri 19	Transcription/Translation Test
Mon 22	DNA Genetic Engineering:Cutting DNA up into pieces, Gluing them back together again in different combinations, and looking to see what happens! Biotechnology tools III: Restriction Enzymes Pallindromes.doc Restriction Enzymes.ppt Plasmids.ppt DNA Scissors Exercise I (hard copy from Dr. F. only)	DNA Gel Electrophoresis CPR DUE MON MAY 6 1 concept (a minimum of 3x150 word paragraphs each) 2 reflections (100 words each min) Typed, 12 point font. double spaced
23	Restriction Enzyme Pre-LAB Lambda DNA Seq.doc Find digest sites for Hind III, Eco RI, and Bam HI and estimate fragment sizes.	Hind III Eco RI Sal I Sma I Pst I Bam HI
24	Enzyme data dump...fragments released? Biotechnology tools IV: DNA Gel Electrophoresis Restriction Enzyme LAB Set up digests, Pour 1% w/v agarose gels Lambda DNA Digestion worksheet.docx DNA "Off to the races" activity (paper only-no e copy)
25	Run Gel and stain/Destain
Fri 26	Collect Data Semi Log Plotting of known Fragment sizes.ppt * To find out the size of unknown fragments * If time: Intro to DNA Restriction Fragment Mapping. Lambda Fragment Semi-log analysis.doc
Mon 29	Plasmids and Restriction DNA mapping Restriction Enzymes.ppt Bacterial Plasmids.ppt DNA scissors exercise II Try mapping an example by Mr. Fowler.
30	Plasmids and Restriction DNA mapping Cont. RFLPs and SNPs (DNA mutations that create or destroy restriction sites) RFLP.ppt RFLP Concept.doc Elephant DNA activity (paper copy only)
May 1	Who Dun It: RFLP Electrophoresis Murder mystery? Set up the digest and the gel.
2	Run and stain the "murder" gel DOG DNA results worksheet.docx
Fri 3	Whodunnit Results Work on CPR
Mon 6	DNA Electrophoresis CPR Due Sequencing DNA	Genetic Engineering CPR DUE FRI MAY 24th! 1 concept (a minimum of 3x150 word paragraphs each) 2 reflections (100 words each min) Typed, 12 point font. double spaced
7	Sequencing DNA	SKIP: Bacterial Hosts Article.doc Bacterial Hosts Article Questions.doc
8	Genetic Engineering.ppt Student Notes scaffold.doc Paper Plasmids
9	Paper Plasmids
Fri 10	Paper Plasmids Clone the Insulin fragment into pAMPKAN
Mon 13	Biotechnology tools V: Bacterial Transformation Transformation pre-assessment Pre-lab (Set up Plates, Tubes, E.coli)
14	Transforming pBlu intoE. coli Perform Transformation (to o/n on ice step.)
15	Plate Cells and incubate o/n Genetic Engineering CPR DUE FRI MAY 24th!	After today I will no longer accept latework up to this date.
16	Calculate transformation frequencies (cfu/ug) Replica-Plate transformants onto Xgal plates Start HB101 for pGLO LAB
Fri 17	pGLO LAB- Transform plasmid, incubate o/n at 4oC Take a look at Xgal plates, pGLO Prelab
Mon 20	State Testing till 10:30. Per 1, 2, 3 only 10:35 start
21	State Testing till 10:30. Per 4, 5, 6 only 10:35 start pGLO LAB- Transform plasmid and plate pGLO tf mix. Take a look at Xgal plates,
22	State Testing till 10:30. Per 1, 2, 3 only 10:35 start
23	Arabinose induction of pGLO CPR time.
Fri 24	State Testing till 10:30. Per 4, 5, 6 only 10:35 start Check for GFP What is a plasmid notes.doc Genetic Engineering CPR DUE
Mon 27	Memorial Day - No School
28	Review Practice Questions and Key.doc Plasmid mapping Qs and answers.pdf
29	Genetic Engineering TEST
30	PCR DNAi Video: https://www.youtube.com/watch?v=2KoLnIwoZKU a good "how does it work" video: https://www.youtube.com/watch?v=DkT6XHWne6E Warning....This is a read-only video (no sound) Bio-Rad Videos: https://www.youtube.com/watch?v=x5yPkxCLads https://www.youtube.com/watch?v=CQEaX3MiDow PCR Webquest.doc PCR Prelab
Fri 31	PCR Human Cheek cell DNA LAB Genetic analysis of the PTC-tasting allele TAS2R38 Step 1. Isolate DNA A general PCR Lab protocol and theory.pdf TAS2R38 PCR Lab Worksheet.docx PCR WebQuest.doc
Mon June 3	Step 2: Setup PCR Cary on with Webquest
4	Step 3: Perform restriction Digest on DNA. Setup and submarine 2% Gel
5	Step 4: Run Gel and stain
6	Destain and Finish WebQuest and Lab Worksheet. Antibodies.ppt Antibodies Vocabulary.doc
Fri 7	Make an antibody model How ELISAs work (Diagram).JPG How ELISAs work (Diagram Key).JPG
Mon 10	Antibodies and disease: The Ebola scenario
11	Start Ebola ELISA
12	Finish Ebola ELISA Epidemiology in action(Epidemiology is the branch of medicine that deals with the incidence, distribution, and possible control of diseases and other factors relating to health.)
13	Goodbye Seniors ?
Fri 14	?
Mon 17	Finals
18	Finals
19	Finals
20	Last Day