DATE |
CLASS ACTIVITY |
ASSIGNMENTS |
Mon Feb 4 |
Semester 2 Start
SNOW DAY.
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5 |
Introduction to Class.
Class Info and Expectations.doc
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3 Ring Binder for classroom ASAP
Prokaryotic vs Eukaryotic Assignment.doc
Due Friday at the beginning of class
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6 |
Who are you: Introductions
Hartman Personality profile.doc
Colors decoded.doc |
7 |
UNIT 1 : DNA Structure
Answer the Q. What is DNA?
What is Biotechnology.ppt
Intro to DNA Webquest.doc
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Fri Feb 8 |
Pro v Euk assignment due
DNA Carries information...Some Notes:
DNA is the information Molecule.ppt
Student notes sheet.doc
Start DNA model building.
Early release for Snomageddon |
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Mon Feb 11 |
SNOWMAGEDDON cont.
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12 |
SNOWMAGEDDON cont.
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13 |
SNOWMAGEDDON cont.
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14 |
SNOWMAGEDDON cont.
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Fri 15 |
SNOWMAGEDDON cont.
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Mon Feb 18 |
Presidents Day |
Tues 19 |
DNA is the information Molecule.ppt
DNA Model Cont.
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20 |
Finish Notes
Draw the carbon backbone of Deoxyribose.
5', 3', and Antiparallel explained
DNA Dance.doc
Finish Model |
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21 |
The Structure of DNA.ppt
The Structure of DNA.notes
DNA Structure readymade notes.doc
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Fri 22 |
Finish Notes
DNA Structure Quick Quiz.doc
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Mon 25 |
DNA Structure Test |
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26 |
Math in the LAB .doc (Yes Math!!!) |
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27 |
Biotechnology Tools I: Micropipettes
Introduction to Micropipettes
Micropipettes 1: Measure for Measure LAB
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28 |
Finish Measure for measure |
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Fri Mar 1 |
Micropipettes 2: Color Wheel LAB
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Micropipette CPR Rubric.doc
CPR example on Gravity written by Dr. F.docx --Due Wed. Mar 13
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Mon Mar 4 |
Micropipettes 3: Micropipette Challenge LAB
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5
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Finish Challenge LAB |
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6 |
Micropipettes 4: Serial Dilutions Lab
Serial Dilution Worksheet.doc
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7 |
SNOW DAY :-(
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Fri 8 |
Serial Dilution and Worksheet |
Paid Summer Internship at the PNW Salmon Center (Belfair) |
Mon 11 |
Pre Lab for Bacterial Serial Dilution
Finish Worksheet |
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12 |
Bacterial Serial Dilution
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13 |
CPR DUE
Bacteria Counting and Data analysis |
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14 |
Unit 2: DNA Function Part 1... DNA functions to replicate itself.
DNA Replication.ppt
DNA Replication Notes scaffold.doc
DNA Replication Picture.doc
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Concept I: DNA replication is Semi Conservative
Last Chance to Finish up all LABS and worksheets
Bozeman on the Meselson and Stahl experiment: Video |
Fri 15 |
DNA Replication Notes |
Concept I: DNA replication is Semi Conservative.
Concept II: Replication always proceeds from a 5'->3' direction
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Mon 18 |
Finish DNA Replication |
Concept III : Replication occurs simultaneously from multiple origins.
Concept IV: Replication requires many enzymes.
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19 |
DNA Extraction LAB |
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20 |
Finish DNA Extraction LAB.
Review DNA Replication Quiz.doc
Quiz Key.doc |
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21 |
DNA Replication Test |
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Fri 22 |
Start Electrophoresis explorationI |
Electrophoresis CPR DUE
1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
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Mon 25 |
Finish Electrophoresis Exploration I |
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26 |
Electrophoresis exploration II
Practice Pouring and Loading gels |
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27 |
Electrophoresis exploration II
Practice Pouring, Loading gels, and sample set-up
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28 |
Run Gel and get results |
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Fri 29 |
Catch up, CPR |
No missing work (including re-writes or Lab worksheets) will be accepted after Today!
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Mon. April 1st -
Fri 5th |
SPRING BREAK |
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Mon April 8 |
Candy Lab |
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9 |
Candy Lab |
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10 |
Electrophoresis CPR DUE
DNA Function 2: Carrying the Code
Part I: Elements of Transcription.
Q. What is a gene?
Introduction to Transcription.ppt
Transcription Notes & Worksheet.doc
Transcription Vocabulary.doc
Complete Lambda DNA Sequence.doc |
|
11 |
Finish Elements of Transcription
RNA and Transcription.ppt
Transcription animation.ppt
Animation Notes.doc
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DNAi Transcription Video
Scribe and Chef analogy video |
Fri 12 |
Part II: Elements of Translation.
Introduction to translation.ppt
the code of translation.ppt
Translation animation.ppt
student animation notes scaffold.doc
Student translation vocab.doc
Translation notes/practice 1.doc
Translation Notes/practice 2.doc
http://www.youtube.com/watch?v=H5udFjWDM3E
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Mon 15 |
FINISH Translation notes & practice worksheets |
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16 |
Dingo
Secret message.doc
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17 |
DNA Mutations.ppt
Mutations practice.doc |
No Late Work after today
Bozeman on Mutations Video
Bozeman Mutation Quiz.docx
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18 |
Review
Ribosome and transcription Practice Diagram.doc
DNA Function Vocab Quiz.doc
DNA Function II - Complete Vocab with Definitions.doc
Transcription/translation practice Quiz.doc
Transcription/translation practice Test.pdf
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Paul Berg Translation Dance Video
Twas Brillig Lyrics.doc
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Fri 19 |
Transcription/Translation Test |
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Mon 22 |
DNA Genetic Engineering:Cutting DNA up into pieces, Gluing them back together again in different combinations, and looking to see what happens!
Biotechnology tools III: Restriction Enzymes
Pallindromes.doc
Restriction Enzymes.ppt
Plasmids.ppt
DNA Scissors Exercise I (hard copy from Dr. F. only)
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DNA Gel Electrophoresis CPR DUE MON MAY 6
1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
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23 |
Restriction Enzyme Pre-LAB
Lambda DNA Seq.doc
Find digest sites for Hind III, Eco RI, and Bam HI and estimate fragment sizes.
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Hind III
Eco RI
Sal I
|
24 |
Enzyme data dump...fragments released?
Biotechnology tools IV: DNA Gel Electrophoresis
Restriction Enzyme LAB
Set up digests, Pour 1% w/v agarose gels
Lambda DNA Digestion worksheet.docx
DNA "Off to the races" activity (paper only-no e copy)
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25 |
Run Gel and stain/Destain
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Fri 26 |
Collect Data
Semi Log Plotting of known Fragment sizes.ppt
* To find out the size of unknown fragments
* If time: Intro to DNA Restriction Fragment Mapping.
Lambda Fragment Semi-log analysis.doc
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Mon 29 |
Plasmids and Restriction DNA mapping
Restriction Enzymes.ppt
Bacterial Plasmids.ppt
DNA scissors exercise II
Try mapping an example by Mr. Fowler.
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30 |
Plasmids and Restriction DNA mapping Cont.
RFLPs and SNPs (DNA mutations that create or destroy restriction sites)
RFLP.ppt
RFLP Concept.doc
Elephant DNA activity (paper copy only)
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May 1 |
Who Dun It:
RFLP Electrophoresis Murder mystery?
Set up the digest and the gel.
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2 |
Run and stain the "murder" gel
DOG DNA results worksheet.docx
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Fri 3 |
Whodunnit Results
Work on CPR |
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Mon 6 |
DNA Electrophoresis CPR Due
Sequencing DNA
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Genetic Engineering CPR DUE
FRI MAY 24th!
1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
|
7 |
Sequencing DNA
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SKIP: Bacterial Hosts Article.doc
Bacterial Hosts Article Questions.doc
|
8 |
Genetic Engineering.ppt
Student Notes scaffold.doc
Paper Plasmids
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9 |
Paper Plasmids
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Fri 10 |
Paper Plasmids
Clone the Insulin fragment into pAMPKAN
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Mon 13 |
Biotechnology tools V: Bacterial Transformation
Transformation pre-assessment
Pre-lab (Set up Plates, Tubes, E.coli)
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14 |
Transforming pBlu intoE. coli
Perform Transformation (to o/n on ice step.) |
|
15 |
Plate Cells and incubate o/n
Genetic Engineering CPR DUE
FRI MAY 24th!
|
After today I will no longer accept latework up to this date. |
16 |
Calculate transformation frequencies (cfu/ug)
Replica-Plate transformants onto Xgal plates
Start HB101 for pGLO LAB |
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Fri 17 |
pGLO LAB- Transform plasmid, incubate o/n at 4oC
Take a look at Xgal plates,
pGLO Prelab
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Mon 20 |
State Testing till 10:30. Per 1, 2, 3 only 10:35 start |
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21 |
State Testing till 10:30. Per 4, 5, 6 only 10:35 start
pGLO LAB- Transform plasmid and plate pGLO tf mix.
Take a look at Xgal plates,
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22 |
State Testing till 10:30. Per 1, 2, 3 only 10:35 start
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23 |
Arabinose induction of pGLO
CPR time. |
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Fri 24 |
State Testing till 10:30. Per 4, 5, 6 only 10:35 start
Check for GFP
What is a plasmid notes.doc
Genetic Engineering CPR DUE |
|
Mon 27 |
Memorial Day - No School |
|
28 |
Review
Practice Questions and Key.doc
Plasmid mapping Qs and answers.pdf |
|
29 |
Genetic Engineering TEST
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|
30 |
PCR DNAi Video:
https://www.youtube.com/watch?v=2KoLnIwoZKU
a good "how does it work" video:
https://www.youtube.com/watch?v=DkT6XHWne6E
Warning....This is a read-only video (no sound)
Bio-Rad Videos:
https://www.youtube.com/watch?v=x5yPkxCLads
https://www.youtube.com/watch?v=CQEaX3MiDow
PCR Webquest.doc
PCR Prelab
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Fri 31 |
PCR Human Cheek cell DNA LAB
Genetic analysis of the PTC-tasting allele TAS2R38
Step 1. Isolate DNA
A general PCR Lab protocol and theory.pdf
TAS2R38 PCR Lab Worksheet.docx
PCR WebQuest.doc
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Mon June 3 |
Step 2: Setup PCR
Cary on with Webquest |
|
4 |
Step 3: Perform restriction Digest on DNA.
Setup and submarine 2% Gel
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5 |
Step 4: Run Gel and stain |
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6 |
Destain and Finish WebQuest and Lab Worksheet.
Antibodies.ppt
Antibodies Vocabulary.doc
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Fri 7 |
Make an antibody model
How ELISAs work (Diagram).JPG
How ELISAs work (Diagram Key).JPG
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Mon 10 |
Antibodies and disease: The Ebola scenario
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11 |
Start Ebola ELISA
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12 |
Finish Ebola ELISA
Epidemiology in action(Epidemiology is
the branch of medicine that deals with the incidence, distribution, and possible control of diseases and other factors relating to health.)
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13 |
Goodbye Seniors
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Fri 14 |
? |
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Mon 17 |
Finals |
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18 |
Finals |
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19 |
Finals |
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20 |
Last Day |
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