Biotech Spring 2019

Mon Feb 4 Semester 2 Start


5 Introduction to Class.

Class Info and Expectations.doc

3 Ring Binder for classroom ASAP

Prokaryotic vs Eukaryotic Assignment.doc

Due Friday at the beginning of class

6 Who are you: Introductions

Hartman Personality profile.doc

Colors decoded.doc
7 UNIT 1 : DNA Structure

Answer the Q
. What is DNA?

What is Biotechnology.ppt

Intro to DNA Webquest.doc

Fri Feb 8 Pro v Euk assignment due

DNA Carries information...Some Notes:

DNA is the information Molecule.ppt

Student notes sheet.doc

Start DNA model building.

Early release for Snomageddon

Mon Feb 11






Mon Feb 18 Presidents Day
 Tues 19 DNA is the information Molecule.ppt

DNA Model Cont.

20 Finish Notes

Draw the carbon backbone of Deoxyribose.

5',  3', and Antiparallel explained

DNA Dance.doc

Finish Model

21 The Structure of DNA.ppt

The Structure of DNA.notes

DNA Structure readymade notes.doc

Fri 22 Finish Notes

DNA Structure Quick Quiz.doc
Mon 25 DNA Structure Test
26 Math in the LAB .doc (Yes Math!!!)  

Biotechnology Tools I: Micropipettes

Introduction to Micropipettes

Micropipettes 1: Measure for Measure LAB

28 Finish Measure for measure
Fri Mar 1 Micropipettes 2: Color Wheel LAB
Micropipette CPR Rubric.doc

CPR example on Gravity written by Dr. F.docx --Due  Wed. Mar 13

Mon Mar 4 Micropipettes 3: Micropipette Challenge LAB
5 Finish Challenge LAB  

Micropipettes 4: Serial Dilutions Lab

Serial Dilution Worksheet.doc



Fri 8 Serial Dilution and Worksheet Paid Summer Internship at the PNW Salmon Center (Belfair)
Mon 11 Pre Lab for Bacterial Serial Dilution 

Finish Worksheet
12 Bacterial Serial Dilution

Bacteria Counting and Data analysis

Unit 2: DNA Function Part 1... DNA functions to replicate itself.

DNA Replication.ppt

DNA Replication Notes scaffold.doc

DNA Replication Picture.doc

Concept I: DNA replication is Semi Conservative

Last Chance to Finish up all LABS and worksheets

Bozeman on the Meselson and Stahl experiment:  Video
Fri 15 DNA Replication Notes Concept I: DNA replication is Semi Conservative.

Concept II: Replication always proceeds from a 5'->3' direction

Mon 18 Finish DNA Replication Concept III : Replication occurs simultaneously from multiple origins.

Concept IV: Replication requires many enzymes.

19 DNA Extraction LAB  
20 Finish DNA Extraction LAB.

Review DNA Replication Quiz.doc

Quiz Key.doc
21 DNA Replication Test  
Fri 22  Start Electrophoresis explorationI Electrophoresis CPR DUE 

1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)

Typed, 12 point font. double spaced

Mon 25 Finish Electrophoresis Exploration I  
 26 Electrophoresis exploration II

Practice Pouring and Loading gels
 27 Electrophoresis exploration II

Practice Pouring, Loading gels, and sample set-up

 28 Run Gel and get results  
 Fri 29 Catch up, CPR No missing work (including re-writes or Lab worksheets) will be accepted after Today!
Mon. April 1st -
Fri 5th
Mon April 8
Candy Lab
 9 Candy Lab  
 10 Electrophoresis CPR DUE

DNA Function 2: Carrying the Code

Part I: Elements of Transcription.

Q. What is a gene?

Introduction to Transcription.ppt

Transcription Notes & Worksheet.doc

Transcription Vocabulary.doc

Complete Lambda DNA Sequence.doc
 11 Finish Elements of Transcription

RNA and Transcription.ppt

Transcription animation.ppt

Animation Notes.doc

DNAi Transcription Video

Scribe and Chef analogy video
 Fri 12  
Part II: Elements of Translation.

Introduction to translation.ppt

the code of translation.ppt

Translation animation.ppt

student animation notes scaffold.doc

Student translation vocab.doc

Translation notes/practice 1.doc

Translation Notes/practice 2.doc

 Mon 15 FINISH Translation notes & practice worksheets  


Secret message.doc

 17 DNA Mutations.ppt

Mutations practice.doc
 No Late Work after today

Bozeman on Mutations Video

Bozeman Mutation Quiz.docx

 18 Review

Ribosome and transcription Practice Diagram.doc

DNA Function Vocab Quiz.doc

DNA Function II - Complete Vocab with Definitions.doc

Transcription/translation practice Quiz.doc

Transcription/translation practice Test.pdf

Paul Berg Translation Dance Video

Twas Brillig Lyrics.doc

 Fri 19  Transcription/Translation Test  
 Mon 22 DNA Genetic Engineering:Cutting DNA up into pieces, Gluing them back together again in different combinations, and looking to see what happens!

Biotechnology tools III: Restriction Enzymes


Restriction Enzymes.ppt


DNA Scissors Exercise I (hard copy from Dr. F. only)
DNA Gel Electrophoresis CPR DUE MON MAY 6

1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)

Typed, 12 point font. double spaced


Restriction Enzyme Pre-LAB

Lambda DNA Seq.doc

Find digest sites for Hind III, Eco RI, and Bam HI and estimate fragment sizes.
 Hind-III-cutsite_1Hind III

EcoR-I-cutsite_1Eco RI

Sal-I-cutsite_1Sal I

Sma-I-cutsite_1Sma I

Pst-I-cutsite_1Pst I

BamH-I-cutsite_1Bam HI

Enzyme data dump...fragments released?

Biotechnology tools IV: DNA Gel Electrophoresis

Restriction Enzyme LAB

Set up digests, Pour 1% w/v agarose gels

Lambda DNA Digestion worksheet.docx

DNA "Off to the races" activity (paper only-no e copy)

 25 Run Gel and stain/Destain
 Fri 26 Collect Data

Semi Log Plotting of known Fragment sizes.ppt

* To find out the size of unknown fragments
* If time: Intro to DNA Restriction Fragment Mapping.

Lambda Fragment Semi-log analysis.doc
 Mon 29  

Plasmids and Restriction DNA mapping

Restriction Enzymes.ppt

Bacterial Plasmids.ppt

DNA scissors exercise II

Try mapping an example by Mr. Fowler.


Plasmids and Restriction DNA mapping Cont.

RFLPs and SNPs (DNA mutations that create or destroy restriction sites)


RFLP Concept.doc

Elephant DNA activity (paper copy only)

 May 1  

Who Dun It:

RFLP Electrophoresis Murder mystery?

Set up the digest and the gel.

 2 Run and stain the "murder" gel

DOG DNA results worksheet.docx
 Fri 3 Whodunnit Results

Work on CPR
 Mon 6 DNA Electrophoresis CPR Due

Sequencing DNA

Genetic Engineering CPR DUE
FRI MAY 24th!

1 concept (a minimum of 3x150 word paragraphs each)
2 reflections (100 words each min)

Typed, 12 point font. double spaced


Sequencing DNA

SKIP: Bacterial Hosts Article.doc

Bacterial Hosts Article Questions.doc

 8 Genetic Engineering.ppt

Student Notes scaffold.doc

Paper Plasmids

 9 Paper Plasmids
 Fri 10 Paper Plasmids

Clone the Insulin fragment into pAMPKAN
 Mon 13
Biotechnology tools V: Bacterial Transformation

Transformation pre-assessment

Pre-lab (Set up Plates, Tubes, E.coli)

 14 Transforming pBlu intoE. coli

Perform Transformation (to o/n on ice step.)
 15 Plate Cells and incubate o/n

Genetic Engineering CPR DUE
FRI MAY 24th!
 After today I will no longer accept latework up to this date.
 16 Calculate transformation frequencies (cfu/ug)

Replica-Plate transformants onto Xgal plates

Start HB101 for pGLO LAB
 Fri 17

pGLO LAB- Transform plasmid, incubate o/n at 4oC

Take a look at Xgal plates,

pGLO Prelab

 Mon 20 State Testing till 10:30.  Per 1, 2, 3 only 10:35 start  
 21 State Testing till 10:30.  Per 4, 5, 6 only 10:35 start

pGLO LAB- Transform plasmid and plate pGLO tf mix.

Take a look at Xgal plates,

 22 State Testing till 10:30.  Per 1, 2, 3 only 10:35 start
 23 Arabinose induction of pGLO

CPR time.
 Fri 24 State Testing till 10:30.  Per 4, 5, 6 only 10:35 start

Check for GFP

What is a plasmid notes.doc

Genetic Engineering CPR DUE
 Mon 27 Memorial Day - No School  
 28 Review

Practice Questions and Key.doc

Plasmid mapping Qs and answers.pdf
Genetic Engineering TEST

 30 PCR DNAi Video:

a good "how does it work" video:
Warning....This is a read-only video (no sound)

Bio-Rad Videos:

PCR Webquest.doc

PCR Prelab

 Fri 31

PCR Human Cheek cell DNA LAB

Genetic analysis of the PTC-tasting allele TAS2R38

Step 1.  Isolate DNA

A general PCR Lab protocol and theory.pdf

TAS2R38 PCR Lab Worksheet.docx

PCR WebQuest.doc

 Mon June 3 Step 2:  Setup PCR

Cary on with Webquest
 4 Step 3:  Perform restriction Digest on DNA.

Setup and submarine 2% Gel
 5 Step 4:  Run Gel and stain  
 6 Destain and Finish WebQuest and Lab Worksheet.


Antibodies Vocabulary.doc

 Fri 7

Make an antibody model

How ELISAs work (Diagram).JPG

How ELISAs work (Diagram Key).JPG

 Mon 10 Antibodies and disease: The Ebola scenario
 11 Start Ebola ELISA
 12 Finish Ebola ELISA

Epidemiology in action(Epidemiology is
the branch of medicine that deals with the incidence, distribution, and possible control of diseases and other factors relating to health.)
 13 Goodbye Seniors

 Fri 14 ?  
 Mon 17  Finals  
 18  Finals  
 19  Finals  
 20  Last Day  
Micropipettes 1: Measure for Measure LAB

Antibodies and protein purification and detection:


Antibodies Vocabulary Worksheet.doc

After today I will no longer accept latework up to this date.