| Date |
Class Activity |
Assignments |
| Mon. Feb 1 |
Semester 2 Start.
Introduction to Class.
Class Info and Expectations.doc
|
3 Ring Binder for classroom ASAP
Prokaryotic vs Eukaryotic Assignment.doc
Due Thursday at the beginning of class.
|
| Tues Feb 2. |
Who are you: Introductions
Hartman Personality profile.doc
Profile worksheet.doc
Profile answer key.doc |
|
| Wed Feb 3 |
UNIT 1 : DNA Structure
Answer the Q. What is DNA?
What is Biotechnology.ppt
Intro to DNA Webquest.doc |
|
| Thurs. Feb 4 |
Pro vs Eukaryotic paper due
DNA Carries information...Some Notes:
DNA is the information Molecule.ppt
Student notes sheet.doc
Start DNA model building.
|
|
| Fri Feb 5 |
DNA is the info molecule Notes.
Continue with DNA Model |
Fact vs. Concept assignment.doc
DUE nextThursday
|
| Mon Feb 8 |
Finish the notes.
continue model building
|
|
| Tues Feb 9 |
What is the 5' and 3' thingy?
The DNA Dance.doc
Complete DNA Model |
Finish DNA Model at home.
3D DNA model to play with at home :-)
http://www.umass.edu/molvis/tutorials/dna/dnapairs.htm
|
| Wed Feb 10 |
The Structure of DNA Webquest.doc |
|
| Thurs Feb 11 |
The Structure of DNA. ppt
DNA Structure Notes.doc
DNA Structure quick review/quiz.doc
Fact v Concept DUE
|
DNA Quick Notes.doc |
| Fri Feb 12 |
NON STUDENT DAY |
|
| Mon Feb 15 |
PRESIDENTS DAY |
|
| Tues Feb 16 |
DNA StructureTEST (including evidence for carying genetic information) |
|
| Wed Feb 17 |
Math in the LAB .doc(Yes Math!!!) |
|
| Thurs Feb 18 |
Biotechnology Tools I: Micropipettes
Introduction to Micropipettes
Micropipettes 1: Measure for Measure LAB
|
|
| Fri Feb 19 |
Micropipettes 1: Measure for Measure LAB |
Micropipette CPR Rubric.doc
CPR example on Gravity written by Dr. F.docx
DUEFri MAR 4 (2 weeks)
|
| Mon Feb 22 |
Micropipettes 2: Color Wheel LAB |
|
| Tues Feb 23 |
Micropipettes 3: Micropipette Challenge LAB |
|
| Wed Feb 24 |
Finish Challenge LAB
|
Paid Research internship at ISB Seattle.pdf
For Juniors only |
| Thurs Feb 25 |
Micropipettes 4: Serial Dilutions Lab
Serial Dilution Worksheet.doc
|
|
| Fri Feb 26 |
Finish and catch-up day
Possible Pre Lab for Bacterial Serial Dilution. |
|
| Mon Feb 29 |
Bacterial Serial Dilution.
Serial Dilution and titration. Counting Bacteria in culture.
Bacterial Serial Dilution and Plating
|
|
| Tues Mar 1 |
Bacteria Counting and Data analysis |
|
| Wed Mar 2 |
Unit 2: DNA Function Part 1... DNA functions to replicate itself.
DNA Replication.ppt
DNA Replication Notes scaffold.doc
DNA Replication Picture.doc
|
Concept I: DNA replication is Semi Conservative
Last Chance to Finish up all LABS and worksheets
Bozeman explains the Meselson and Stahl experiment:
https://www.youtube.com/watch?v=JcUQ_TZCG0w |
| Thurs Mar 3 |
DNA Replication Notes |
Concept I: DNA replication is Semi Conservative
Concept II: Replication always proceeds from a 5'->3' direction
|
| Fri Mar 4 |
Micropipette CPR DUE
Finish DNA Replication
A good Summary: Web Animation:
http://www.johnkyrk.com/DNAreplication.html
|
Concept III : Replication occurs simultaneously from multiple origins.
Concept IV: Replication requires many enzymes. |
| Mon Mar 7 |
DNA Extraction LAB |
|
| Tues Mar 8 |
DNA Extraction LAB
Sample Test Q's for DNA Replication with Key.doc
Quick review Quiz No Key.doc |
|
| Wed Mar 9 |
DNA REPLICATION TEST |
|
| Thurs Mar 10 |
Biotechnology tools II: Electrophoresis
Electrophoresis exploration I Pre-LAB
|
Electrophoresis CPR DUEMon Mar 21
2 concepts (a minimum of 3x100 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
|
| Fri Mar 11 |
No School (or a possible Snow Make-up day) |
|
| Mon Mar 14 |
Electrophoresis exploration I Pre-LAB |
No missing work (including re-writes or Lab worksheets) will be accepted after Thursday! |
| Tues Mar 15 |
Electrophoresis Exploration I Electricity, pH, and Buffers |
|
| Wed Mar 16 |
Electrophoresis exploration IIPractice Pouring and Loading gels |
|
| Thurs Mar 17 |
Electrophoresis exploration IIPractice and sample set-up |
|
| Fri Mar 18 |
Catch up and CPR day |
|
| Mon Mar 21 |
Electrophoresis CPR Due
Electrophoresis exploration II Dye Lab |
|
| Tues Mar 22 |
Candy LAB |
|
| Wed Mar 23 |
Candy LAB |
|
| Thurs Mar 24 |
DNA Function 2: Carrying the Code
Part I: Elements of Transcription.
Q. What is a gene?
Introduction to Transcription.ppt
Transcription Notes & Worksheet.doc
Carrying the code Vocabulary.doc
|
|
| Fri Mar 25 |
Finish Elements of Transcription
RNA and Transcription.ppt
Transcription animation.ppt
Animation Notes.doc
|
|
| Mon Mar 28 |
Part II: Elements of Translation.
Introduction to translation.ppt
the code of translation.ppt
Translation animation.ppt
student animation notes scaffold.doc
Student translation vocab.doc
Translation notes/practice 1.doc
Translation Notes/practice 2.doc
http://www.youtube.com/watch?v=H5udFjWDM3E
|
|
| Tues Mar 29 |
FINISH Translation notes & practice worksheets
|
|
| Wed Mar 30 |
Dingo
Paul Berg Translation DanceVideo:
Twas Brillig Lyrics.doc
http://www.youtube.com/watch?v=WTRmvnlNVw4
DNA Function Vocab Quiz.doc
DNA Function II - Complete Vocab with Definitions.doc
Secret message.doc
Ribosome and transcription Practice Diagram.doc
|
|
| Thurs Mar 31 |
From Gene to Protein Review packet.docx |
|
| Fri April 1 |
From Gene to Protein Review packet.docx |
After today I will no longer accept latework up to this date. |
|
Spring Break |
|
| Mon April 11 |
There are 6 possible reading frames
Mutations in the code.ppt
Notes scaffold (hard copy only)
Mutation Practice Worksheet.doc
|
|
| Tues April 12 |
Review
Transcription and Translation practice Quiz + KEY.doc
Ribosome and Transcription complex naming diagram and key.doc
Vocab quiz.doc |
DNA Function II - Complete Vocab with Definitions.doc
Crash Course video on DNA->RNA->Protein
https://www.youtube.com/watch?v=itsb2SqR-R0
Bozeman on Transcription and Translation:
https://www.youtube.com/watch?v=h3b9ArupXZg
(Dont wory about RNA processing or endoplasmic reticulum!)
Bozeman Transcription and Translation Quiz.doc
Bozeman on Mutations :https://www.youtube.com/watch?v=eDbK0cxKKsk
Bozeman Mutations Quiz.doc
|
| Wed April 13 |
DNA FUNCTION TEST |
|
| Thurs April 14 |
DNA Genetic Engineering:Cutting DNA up into pieces, Gluing them back together again in different combinations, and looking to see what happens!
Biotechnology tools III: Restriction Enzymes
Pallindromes.doc
Restriction Enzymes.ppt
Bacterial Plasmids.ppt (Slides 4, 5, and 6)
DNA Scissors Exercise I (hard copy from Dr. F. only)
|
DNA Gel Electrophoresis CPR DUEin a couple of weeks
2 concepts (a minimum of 3x100 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
|
| Fri April 15 |
Finish DNA Scissors activity.
Restriction Enzyme Pre-LAB
Lambda DNA Seq.doc
Find digest sites for 6-8 enzymes |
|
| Mon April 18 |
Enzyme data dump...fragments released?
Biotechnology tools IV: DNA Gel Electrophoresis
Restriction Enzyme LAB
Set up digests
Lambda DNA Digestion worksheet.docx
DNA "Off to the races" activity (paper only-no e copy)
|
|
| Tues April 19 |
Run Gel and stain/Destain |
|
| Wed April 20 |
Collect Data
Semi Log Plotting of known Fragment sizes.ppt
* To find out the size of unknown fragments
* If time: Intro to DNA Restriction Fragment Mapping.
Lambda Fragment Semi-log analysis.doc |
|
| Thurs April 21 |
Plasmids and Restriction DNA mapping
Restriction Enzymes.ppt
Bacterial Plasmids.ppt (Slides 4, 5, and 6)
DNA scissors exercise II
Try mapping an example by Mr. Fowler.
|
|
| Fri April 22 |
Plasmids and Restriction DNA mapping Cont.
RFLPs and SNPs (DNA mutations that create or destroy restriction sites)
RFLP.ppt
RFLP Concept.doc
Elephant DNA activity (paper copy only)
|
RFLP analysis and Kissing
http://www.bbc.com/news/health-30055646
|
| Mon April 25 |
Who Dun It: RFLP Electrophoresis Murder mystery?
Set up the digest and the gel.
|
|
| Tues April 26 |
Run and stain the "murder" gel
DOG DNA results worksheet.docx |
DNA Gel Electrophoresis CPR DUE Mon May 2
2 concepts (a minimum of 3x100 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
|
| Wed April 27 |
Whodunnit Results
Sequencing DNA |
|
| Thurs April 28 |
Bacterial Hosts Article.doc
Bacterial Hosts Article Questions.doc
|
|
| Fri April 29 |
Sequencing DNA
Genetic Engineering.ppt
Student Notes scaffold.doc
|
Genetic Engineering CPR DUEMay 23
2 concepts (a minimum of 3x100 word paragraphs each)
2 reflections (100 words each min)
Typed, 12 point font. double spaced
|
| Mon May 2 |
DNA Electrophoresis CPR DUE
Sequencing DNA |
|
| Tues May 3 |
Sequencing DNA
Paper Plasmids |
|
| Wed May 4 |
Paper Plasmids |
|
| Thurs May 5 |
Clone the Insulin fragment into pAMPKAN
Biotechnology tools V: Bacterial Transformation
Transformation pre-assessment
|
|
| Fri May 6 |
Pre-LAB (prep plates/bacteria/set up tubes) |
|
| Mon May 9 |
Transforming pBlu intoE. coli
Perform Transformation (to o/n on ice step.) |
|
| Tues May 10 |
Plate Cells and incubate o/n |
After today I will no longer accept latework up to this date. |
| Wed May 11 |
Calculate transformation frequencies (cfu/ug)
Replica-Plate transformants onto Xgal plates
Start HB101 for pGLO LAB |
|
| Thurs May 12 |
pGLO LAB- Transform plasmid, incubate o/n at 4oC
Take a look at Xgal plates,
|
|
| Fri May 13 |
plate pGLO transformation mix
pGLO Worksheet.doc
"What is a Plasmid"...Plasmid Questions and answers.doc |
|
| Mon May 16 |
Test UV light on transformants. Add Arabinose to plates.
Control of gene expression. The LacZ operon
Lac operon Notes (complete).doc
Useful Lac Operon Web Links.doc
|
|
| Tues May 17 |
Check transformants for presence of GFP
Review for Test.doc |
|
| Wed May 18 |
Go over Restriction Mapping a Plasmid.pdf |
|
| Thurs May 19 |
Genetic Engineering Test |
|
| Fri May 20 |
PCR DNAi Video:
https://www.youtube.com/watch?v=2KoLnIwoZKU
a good "how does it work" video:
https://www.youtube.com/watch?v=DkT6XHWne6E
Warning....This is a read-only video (no sound)
Bio-Rad Videos:
https://www.youtube.com/watch?v=x5yPkxCLads
https://www.youtube.com/watch?v=CQEaX3MiDow
PCR Webquest.doc
PCR Prelab
|
|
| Mon May 23 |
Genetic Engineering techniques CPR Due Today
PCRHuman Eyebrow cell DNALAB
Genetic analysis of the PTC-tasting allele TAS2R38
Isolate DNA
A general PCR Lab protocol and theory.pdf
TAS2R38 PCR Lab Worksheet.docx
PCR WebQuest.doc
|
|
| Tues May 24 |
PCR WebQuest.doc |
|
| Wed May 25 |
PCRHuman Eyebrow cell DNALAB
Perform FNU Restriction Digest
Set up 2% w/v Agarose gel and submarine overnight in TAE buffer
|
|
| Thurs May 26 |
Set up Gel |
|
| Fri May 27 |
Run Gel/Stain/results
FinishTAS2R38 PCR Lab Worksheet.docx
andPCR WebQuest.doc |
|
| Mon May 30 |
Memorial Day -No School |
|
| Tues May 31 |
Antibodies and protein purification and detection:
Antibodies.ppt
Antibodies Vocabulary Worksheet.doc
|
3D Antibodies:
http://www.umass.edu/molvis/bme3d/materials/explore.html
The scale of a virus and antibody:
http://htwins.net/scale/
|
| Wed June 1 |
Make an antibody model
How ELISAs work (Diagram).JPG
How ELISAs work (Diagram Key).JPG |
|
| Thurs June 2 |
Yearbook Distribution
Finish Diagram
ELISA webquest.doc |
|
| Fri June 3 |
Spirit Week Assembly
Antibodies and disease: The Ebola scenario
Ebola ELISA Pre-LAB |
|
| Mon June 6 |
Senior Finals
Ebola ELISA of Ebola samples
Ebola ELISA instructions and Questions.doc |
|
| Tues June 7 |
Senior Finals
Finish Ebola ELISA
Epidemiology in action(Epidemiology is
the branch of medicine that deals with the incidence, distribution, and possible control of diseases and other factors relating to health.)
SET UP pGLO HB101 Cells for plasmid purification.
|
|
| Wed June 8 |
Seniors Last Day |
|
| Thurs June 9 |
|
|
| Fri June 10 |
2nd.Semester Finals Schedule 2015-16.docx |
|
| Mon June 13 |
2nd.Semester Finals Schedule 2015-16.docx |
|
| Tues June 14 |
2nd.Semester Finals Schedule 2015-16.docx |
|
| Wed June 15 |
LAST DAY
Schedule.xlsx |
|